Viability assays: A comparison of luminescence-, fluorescence-, and absorbance-based assays to determine viable cell counts

There are numerous viability assay kits available which allow cellular viability quantification using absorbance (Abs), fluorescence (FI), or luminescence (Lum) readouts in a microplate reader.

In this application note, BMG Labtech compares three popular representatives (Vybrant^® MTT, alamarBlueTM, and CellTiter-Glo^®) in terms of their usability, as well as lower and upper limit of detection, to support you in the selection of the optimal viability assay for your individual application.